The antibody solution should be stored between 2°C and 8°C
Recommended Usage
For flow cytometric staining, it is recommended to use less than 0.25 ug of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application
Background Information
CD14 is a pattern recognition receptor critical for innate immunity. It exists as a glycosylphosphatidylinositol (GPI)-anchored membrane protein (mCD14) on monocytes, macrophages, and neutrophils, and as a soluble protein (sCD14) in the blood, generated via enzymatic cleavage or shedding. Membrane CD14 acts as a pro-inflammatory co-receptor for lipopolysaccharide (LPS), interacting with LPS-binding protein (LBP) and TLR4 to activate NF-κB and MAPK signaling, driving inflammatory responses. Soluble CD14 enables LPS responsiveness in cells lacking CD14, such as endothelial cells. CD14 expression is modulated by stimuli including LPS, IFNγ, PMA, calcium ionophore, and anti-CD14 antibodies. Elevated sCD14 levels are observed in conditions such as sepsis, systemic lupus erythematosus, HIV infection, common variable immunodeficiency (CVID), nonalcoholic fatty liver disease (NAFLD), and breast invasive ductal carcinoma, highlighting its potential as a biomarker for infection, inflammation, and disease prognosis.
