| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 601003 | 25 ug | $245 | ||
| 601004 | 100 ug | $595 |
| Application | ELISA |
|---|---|
| Format | Liquid, Biotinylated |
| Expression Host | CHO |
| Target Name | Hexosaminidase A, Hexosaminidase B |
| Species | Human |
| Accession Number | P07686/P06865 |
| Sources | Recombinant human HexB (Ala43-Met556) with C-terminus DYKDDDDK tag and human HexA (Leu23-Thr529) with C-terminus His tag are co-expressed in CHO cells. Heterodimer protein is purified sequentially by anti-FLAG tag resin and Nickel resin. The purified heterodimer is then Biotinylated in vitro. |
| Molecular Weight | Human HexA protein has the predicted molecular weight of 60 kD and human HexB protein has the predicted molecular weight of 59.8 kD . Under DTT-reducing conditions, both proteins migrate at approximately 65 kDa on SDS-PAGE. |
| Affinity Tag | C-His and C-DYKDDDDK |
| Purity | >95% based on SDS-PAGE under reducing condition |
| Regulatory Status | RUO |
| Formulation | PBS with 0.09% NaN3, 1% BSA, 10% glycerol (0.2 μm filtered) |
| Endotoxin level | Not tested |
| Protein Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration. |
| Storage and Handling | Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS. For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability. |
Beta-hexosaminidases are lysosomal enzymes that hydrolyze terminal N-acetyl-D-hexosamine residues from GM2 gangliosides and globo-sphingolipids. They exist in three isoforms: Hex A (αβ), Hex B (ββ), and Hex S (αα), formed by different combinations of α and β subunits encoded by the HEXA and HEXB genes. Recombinant HexA-His/HexB-FLAG Heterodimer corresponds to Hex A isoform.
