| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 800203 | 25 ug | $245 | ||
| 800204 | 100 ug | $595 |
| Application | ELISA, BLI |
|---|---|
| Format | Liquid, Biotinylated |
| Expression Host | HEK293 |
| Target Name | CD16A,FCGR3A, FCG3, FCGR3, IGFR3, |
| Species | Human |
| Sources | Human CD16a protein (Accession Number NP_P08637-1, natural variant Val176) (Gly17-Gln208) with C-terminus His tag and Avi tag is expressed in HEK293 cells. This protein was site-specifically labeled with Biotin by BirA ligase. |
| Accession Number | P08637 |
| Molecular Weight | The 227 amino acid protein has a predicted molecular weight of 25.5 kDa. The protein migrates at approximately 40-50 kDa on SDS-PAGE with DTT-reduced conditions. |
| Affinity Tag | C-His-Avi |
| Purity | >95% based on SDS-PAGE under reducing condition |
| Regulatory Status | RUO |
| Formulation | 1xPBS buffer, pH7.4, 0.22 µm filtered |
| Endotoxin level | Less than 0.1 EU/µg protein as determined by the LAL method |
| Protein Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration. |
| Storage and Handling | Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability. |
CD16 is a low-affinity Fc receptor and exists in two isoforms: FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc region of IgG antibodies with distinct tissue-specific expression pattern. CD16a is a transmembrane glycoprotein with intermediate affinity, expressed on natural killer (NK) cells, macrophages, subsets of T cells, immature thymocytes, and placental trophoblasts. In contrast, CD16b is a low-affinity, GPI-anchored receptor expressed primarily on neutrophils and eosinophils. CD16a plays a key role in mediating phagocytosis, antibody-dependent cellular cytotoxicity (ADCC), enzyme secretion, and immune complex clearance.
