| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 811303 | 25 ug | $245 | ||
| 811304 | 100 ug | $595 |
| Application | ELISA, BLI |
|---|---|
| Format | Liquid, Biotinylated |
| Expression Host | CHO |
| Target Name | TNFRSF18, AITR, GITR, CD357 |
| Species | Human |
| Sources | Recombinant Human Human GITR/TNFRSF18 (Gln26-Glu161) with C-terminus His-Avi-tag is expressed in CHO cell. This protein was site-specifically labeled with Biotin by BirA ligase. |
| Accession Number | Q9Y5U5 |
| Molecular Weight | The protein has a predicted molecular weight of 18.1 kDa. Under DTT-reducing conditions, it migrates at approximately 25 kDa on SDS-PAGE. |
| Affinity Tag | C-His-Avi |
| Purity | >95% based on SDS-PAGE under reducing condition |
| Regulatory Status | RUO |
| Formulation | 1xPBS buffer, pH7.4, 0.22 µm filtered |
| Endotoxin level | Less than 0.1 EU/µg protein as determined by the LAL method |
| Protein Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration. |
| Storage and Handling | Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability. |
GITR (glucocorticoid-induced TNFR-related protein), also known as TNFRSF18 or CD357, is a 25 kD member of the TNF receptor superfamily that acts as the receptor for TNFSF18 (GITRL). It is primarily expressed on activated T cells and regulatory T cells and is upregulated upon T cell receptor engagement. GITR plays a key role in immune regulation by influencing T cell proliferation, TCR-mediated apoptosis, and the function of regulatory T cells, thereby contributing to the maintenance of self-tolerance. GITR signaling activates NF-κB via the TRAF2/NIK pathway and interacts with TRAF1–3. It is also implicated in T cell–endothelial cell interactions and the pathogenesis of autoimmune diseases.
