| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 604201 | 25 ug | $79 | ||
| 604202 | 100 ug | $199 |
| Application | FC |
|---|---|
| Format | Liquid, Purified |
| Expression Host | E.coli |
| Target Name | Annexin A5 |
| Species | Human |
| Accession Number | P08758 |
| Sources | Recombinant Human Annexin A5 (Met1-Asp320) with N-His-Xa tag is expressed in E.coli system. His tag is cut by Xa after purification. |
| Molecular Weight | This protein has a predicted molecular weight of 35.9 kDa. Under DTT-reducing conditions, the protein migrates at approximately 35 kDa on SDS-PAGE. |
| Affinity Tag | None |
| Purity | >95% based on SDS-PAGE under reducing condition |
| Regulatory Status | RUO |
| Formulation | 1xPBS/0.09%NaN3 |
| Endotoxin level | Not tested |
| Protein Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration. |
| Storage and Handling | Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability. |
Annexin A5 is a member of the annexin family, a group of proteins that bind negatively charged phospholipids in a calcium-dependent manner. During apoptosis, phosphatidylserine (PS) translocates to the outer leaflet of the plasma membrane. Annexin A5 specifically binds to this exposed PS, making it a widely used marker for early apoptosis. It is commonly used in combination with propidium iodide (PI) in flow cytometry or microscopy assays
