| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 601901 | 25 ug | $145 | ||
| 601902 | 100 ug | $295 |
| Application | ELISA, BLI |
|---|---|
| Format | Liquid, Purified |
| Expression Host | CHO |
| Target Name | SURF1, Surfeit locus protein 1 |
| Species | Human |
| Accession Number | Q15526 |
| Sources | Recombinant human SURF1 protein (Val98-Val265) with C-terminus Fc tag was expressed in CHO Cells. |
| Molecular Weight | This protein has a predicted molecular weight of 45.3 kDa. Under DTT-reducing conditions, the protein migrates at approximately 50 kDa on SDS-PAGE. |
| Affinity Tag | C-Fc |
| Purity | >90% based on SDS-PAGE under reducing condition |
| Regulatory Status | RUO |
| Formulation | 1xPBS with 5mM DTT, pH 7.4 (0.2 μm filtered) |
| Endotoxin level | Not tested |
| Protein Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration. |
| Storage and Handling | Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability. |
SURF1 is a mitochondrial inner membrane protein encoded by the SURF1 gene. Surf1 involves in the assembly of cytochrome c oxidase (COX), also known as complex IV of the respiratory chain. It contains multiple transmembrane domains and plays a crucial role in stabilizing early COX subunits during complex formation.
Mutations in SURF1 are a major cause of Leigh syndrome, a severe neurodegenerative disorder characterized by progressive brain lesions, motor delays, and mitochondrial dysfunction. These mutations lead to isolated COX deficiency, impairing energy production in high-demand tissues like the brain. SURF1 is therefore critical to mitochondrial function, and its dysfunction is strongly associated with mitochondrial disease.
