The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended Usage
For flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation Laser
Red Laser (633 nm)
Background Information
CD137 is a 39 kDa type I transmembrane protein and a member of the Tumor Necrosis Factor Receptor (TNFR) superfamily. It is expressed on activated T cells, NKT cells, and NK cells, where it functions as a co-stimulatory molecule that promotes T cell proliferation, survival, and monocyte activation through its interaction with 4-1BB ligand. CD137 plays a key role in anti-tumor immunity. Agonistic anti-CD137 antibodies enhance tumor-specific CD8⁺ T cell responses, increase NK and dendritic cell function, and improve antibody-dependent cellular cytotoxicity (ADCC) when used with tumor-targeting monoclonal antibodies. Its expression can be upregulated on NK cells after CD16 ligation, although its effect on NK cell cytotoxicity can vary between human and murine systems. Overall, CD137 is an important immunomodulatory target in cancer immunotherapy.
