The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended Usage
For flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation Laser
Red Laser (633 nm)
Background Information
CD25 is a 55 kDa type I transmembrane glycoprotein and the alpha chain of the high-affinity interleukin-2 receptor (IL-2R). It is expressed on activated T and B cells, progenitor lymphocytes, activated monocytes/macrophages, and a subset of non-stimulated CD4⁺ regulatory T cells (Tregs). CD25 associates with the IL-2 receptor β (CD122) and common γ (CD132) chains to form the functional high-affinity IL-2R complex. Functionally, CD25 is critical for T cell activation, proliferation, and immune regulation. Deficiency of CD25 in mice causes severe autoimmunity and lethal lymphoproliferative disorders, mirroring defects seen in FOXP3 deficiency. In humans, genetic studies and case-control analyses have linked CD25 to autoimmune diseases such as Graves’ disease, often accompanied by elevated soluble IL-2Rα (sIL-2Rα). Its role in immune regulation also makes it a target in certain cancer immunotherapies.
