The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended Usage
For flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation Laser
Red Laser (633 nm)
Background Information
CD69 (also known as CLEC2C, VEA, AIM, EA1, MLR3, gp34/28) is a type II transmembrane C-type lectin glycoprotein that forms a ~60 kDa disulfide-linked homodimer. The mature human protein consists of a 40-amino-acid cytoplasmic domain, a 21-amino-acid transmembrane segment, and a 138-amino-acid extracellular domain containing a C-type lectin domain, sharing ~57% sequence identity with mouse and rat CD69. CD69 is rapidly upregulated on activated immune cells, including CD4⁺ and CD8⁺ T cells, NK cells, NKT cells, γδ T cells, B cells, dendritic cells, neutrophils, and a subset of thymocytes and platelets. It functions as an early activation marker, mediates T cell differentiation, immune cell trafficking, and homing of CD4⁺ T cells and plasmablasts to bone marrow, while inhibiting migration of dermal dendritic cells to lymph nodes.
