Use at concentrations comparable to those used for the target-specific antibody. Suggest to keep at lower than 0.1ug/test/
Excitation Laser
Blue Laser (488 nm) Green/Yellow laser (532/561nm)
Background Information
Immunoglobulin M (IgM) is the largest antibody isotype and the first produced during an initial immune response. Structurally, it forms a pentamer of five immunoglobulin units, giving it a high molecular weight of about 900 kDa and up to ten antigen-binding sites, though not all can engage simultaneously. Because of its size, IgM remains primarily in the serum and is produced largely in the spleen. Rat IgM functions similarly, serving as an early, broad-acting antibody produced by rat B cells to recognize and neutralize pathogens. It is widely used in research—as a control reagent, in diagnostic assays, and in studies of infection and immunity. Various established laboratory methods exist for producing and purifying rat IgM, making it a valuable tool for advancing immunological understanding and supporting the development of new disease treatments
