FITC Anti-HA Tag Antibody

Product Details


Clone12CA5
ApplicationFlow Cytometry
ReactivityHA tag, All Species Expected
FormatFITC
Target NameHA epitope tag, YPYDVPDYA tag, Hemagglutinin tag
IsotypeMouse IgG2b
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.4, with stabilizer
Protein Concentration0.5 mg/mL
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. FITC has an excitation max at 493 nm and an emission max at 525 nm.
Excitation LaserBlue Laser (488 nm) Green/Yellow laser (532/561nm)
See All FormatsClone 12CA5

Background Information


The HA tag (hemagglutinin tag) is a short linear epitope (YPYDVPDYA) derived from amino acids 98–106 of influenza A hemagglutinin. With a molecular weight of approximately 1.1 kDa, it is commonly fused to the N- or C-terminus of recombinant proteins to facilitate detection, localization, and purification when protein-specific antibodies are unavailable. Anti-HA tag antibodies specifically recognize the HA epitope and are widely used in applications such as western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and intracellular flow cytometry to study protein expression, topology, and protein–protein interactions.

Isotype Control


FITC Mouse IgG2b Isotype Control Antibody

Data Sheets


FITC Anti-HA Tag Antibody TDS

Related Protocols


Flow Cytometry Protocol


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