GST Protein with multiple tags

Product Details


ApplicationELISA, BLI
FormatLiquid, Purified
Expression HostE.coli
Target NameGST with multiple tags protein (Myc-Avi-V5-DYKDDDDK-HA-His)
SpeciesSchistosoma japonicum
SourcesRecombinant GST protein with multiple tags (Myc-Avi-V5-FLAG-HA-His) at C-terminus is expressed in E.coli system.
Molecular WeightThis protein has a predicted molecular weight of 35.9 kDa. Under DTT-reducing conditions, the protein migrates at approximately 35 kDa on SDS-PAGE. The smaller band below 30 kDa might correspond to the early translational termination product.
Affinity TagC-Myc-Avi-V5-DYKDDDDK-HA-His
Purity>90% based on SDS-PAGE under reducing condition
Regulatory StatusRUO
Formulation1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin levelNot tested
Protein Concentration25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.

Data Sheets


GST Protein with multiple tags TDS

Related Protocols


Direct ELISA Protocol


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