| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 601801 | 25 ug | $145 | ||
| 601802 | 100 ug | $295 |
| Application | ELISA, BLI |
|---|---|
| Format | Liquid, Purified |
| Expression Host | HEK293 |
| Target Name | SUMF1, Formylglycine-generating enzyme |
| Species | Human |
| Accession Number | Q8NBK3 |
| Sources | Recombinant human SUMF1 protein (Ser34-Asp374) with C-terminus His tag was expressed in 293 Cells. |
| Molecular Weight | This protein has a predicted molecular weight of 38.8 kDa. Under DTT-reducing conditions, the protein migrates at approximately 40-45 kDa on SDS-PAGE. |
| Affinity Tag | C-His |
| Purity | >95% based on SDS-PAGE under reducing condition |
| Regulatory Status | RUO |
| Formulation | 25 mM HEPES, 150 mM NaCl, 10% Glycerol, pH 7.4 |
| Endotoxin level | Not tested |
| Protein Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration. |
| Storage and Handling | Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered HEPES buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability. |
Human SUMF1 (also known as formylglycine-generating enzyme, FGE) is a 42 kDa soluble glycoprotein located in the endoplasmic reticulum (ER) lumen. It activates sulfatases by converting a cysteine residue in their catalytic site into a formylglycine, a modification essential for sulfatase function. SUMF1 can form monomers, disulfide-linked homodimers, or heterodimers with SUMF2. It is a Ca²⁺-binding protein and exists in three splice isoforms. Mature human SUMF1 shares 90% amino acid identity with its mouse counterpart.
