| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 515501 | 1 mg | $160 | ||
| 515502 | 5 mg | $400 | ||
| 515503 | 25 mg | $1100 |
| Clone | H57-597-m2a |
|---|---|
| Application | ELISA, WB, Flow cytometry, IHC, ICC, animal model study |
| Host Species | CHO cells |
| Reactivity | Mouse |
| Format | Liquid |
| Target Name | mouse TCR beta, TCRb |
| Product Description | In Vivo Grade Recombinant Anti-mouse TCR beta Monoclonal Antibody |
| Isotype | Mouse IgG2a |
| Antibody Type | Recombinant |
| Regulatory Status | RUO |
| Purity | >95% by reducing SDS-PAGE |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Storage Conditions | 4ºC |
| Grade | In vivo |
| Recommended Usage | This product is suitable for in vivo animal use. Optimal amounts need to be determined empirically for each experiment. |
| See All Formats | Clone H57-597-m2a |
The mouse T cell receptor (TCR) β chain is a critical component of the heterodimeric αβ TCR complex expressed on most mature T lymphocytes. Together with the TCR α chain, it confers antigen specificity to T cells, enabling the recognition of peptide fragments presented by major histocompatibility complex (MHC) molecules on antigen-presenting cells. This recognition event is essential for initiating adaptive immune responses, including T cell activation, proliferation, and differentiation into effector and memory subsets.
Structurally, the TCR β chain is composed of variable (V), diversity (D), joining (J), and constant (C) gene segments that undergo somatic recombination during T cell development in the thymus. This recombination process generates the highly variable complementarity-determining region 3 (CDR3), which contributes most significantly to antigen specificity. The β chain pairs non-covalently with the TCR α chain, forming the antigen-binding site. Each chain contains two extracellular immunoglobulin-like domains, a variable domain involved in antigen binding and a constant domain that stabilizes structure, along with a transmembrane segment and a short cytoplasmic tail. The TCR complex also associates with CD3 signaling molecules (CD3γ, CD3δ, CD3ε, and CD3ζ), which transduce activation signals through immunoreceptor tyrosine-based activation motifs (ITAMs).
The ligands for the mouse TCR β chain are peptide antigens bound to MHC class I or II molecules. Engagement of the αβ TCR with these peptide-MHC complexes triggers receptor conformational changes that initiate intracellular signaling cascades involving kinases such as Lck and ZAP-70, leading to T cell activation and effector function.
In disease, alterations in TCR β chain expression or repertoire diversity can contribute to immune dysfunction. Restricted TCR β repertoires are associated with autoimmune diseases, such as experimental autoimmune encephalomyelitis (EAE, a model for multiple sclerosis), and with impaired immune defense in infections or cancer. Somatic mutations or skewed TCR β usage have also been observed in T cell lymphomas and leukemia.
The mouse TCR β chain has significant therapeutic and experimental relevance. It serves as a model for studying clonal selection, antigen recognition, and tolerance mechanisms. In immunotherapy, manipulation of TCR β sequence diversity underpins TCR-engineered T cell strategies for cancer and infection control. Furthermore, analyzing mouse TCR β repertoires provides insights into vaccine efficacy, autoimmune mechanisms, and immune reconstitution following bone marrow transplantation.
In Vivo Star Anti-Mouse TCR beta Antibody TDS
Have a product or application question? Consult our FAQs or contact us.
