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Nickel resin

Recombinant proteins of various sizes, with either C-terminal or N-terminal His tags, were efficiently purified using Nickel resin and eluted with 400 mM imidazole in 20 mM Tris, 300 mM NaCl, pH 8.0. Eluted proteins were analyzed by SDS-PAGE under both non-reducing (P–) and reducing (P+) conditions and stained with BlinkBlue SDS-PAGE Staining Buffer for 30 minutes. The protein ladder (from top to bottom) corresponds to 185, 115, 80, 65, 50, 30, and 25 kDa, respectively.

Recombinant proteins of various sizes, with either C-terminal or N-terminal His tags, were efficiently purified using Nickel resin and eluted with 400 mM imidazole in 20 mM Tris, 300 mM NaCl, pH 8.0. Eluted proteins were analyzed by SDS-PAGE under both non-reducing (P–) and reducing (P+) conditions and stained with BlinkBlue SDS-PAGE Staining Buffer for 30 minutes. The protein ladder (from top to bottom) corresponds to 185, 115, 80, 65, 50, 30, and 25 kDa, respectively.

Cat #	Size	Price	Quantity
700301	10mL	$99
700302	50mL	$399

Product Details

Application	Nickel resin is suitable for purifying recombinant proteins with a His tag (minimum 6×His) at either the N- or C-terminus. In principle, it may also bind proteins with internal His tags, though this application has not been validated by the manufacturer. The tested binding capacity is approximately 10–20 mg of His-tagged protein per mL of resin. When starting from culture supernatant, >95% purity can typically be achieved with a single-step purification using Nickel resin.
Product Description	Nickel charged cross-linked 6% agarose. The Nickel resin is supplied as 50% slurry in 20% Ethanol.
User Manual	Nickel Resin Application Guideline (PDF)
Storage and Handling	Store the vial at 4°C (DO NOT FREEZE). The unopened vial is stable for twelve months when stored at 4°C.

Data Sheets

Data Sheets

Nickel Resin Application Guidlines.pdf

Nickel resin TDS