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Recombinant Human Flt3 Ligand Protein

Cat # Size Price Quantity
63040120 µg$250
630402100 µg$625

  Bulk/Custom

Product Details

ApplicationBioassay
FormatLyophilized from sterile PBS, pH 7.4.
Expression HostE.coli
Target NameFlt-3 Ligand, FLT3LG, FMS-related tyrosine kinase 3 ligand
SpeciesHuman
accession numberP49771-1
SourcesA DNA sequence encoding the human FLT3LG (P49771-1) (Thr27-Ala181) was expressed with an N-terminal Met.
Molecular WeightThe recombinant Human FLT3LG consists of 156 amino acids and predicts a molecular mass of 17.75 kDa
Affinity TagNone
Purity≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS(Routinely tested).
Regulatory StatusRUO
Endotoxin level<0.01EU per μg protein
Protein ConcentrationLyophilized
Storage and HandlingProteins are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Research AreasActivated T cells, Dendritic cells, Hematopoietic stem cells

Background Information

Human Fms-like tyrosine kinase 3 ligand (Flt3L) is a hematopoietic growth factor that plays a central role in the development and expansion of immune cells, particularly dendritic cells. It is produced by a variety of cell types, including stromal cells and activated T cells, and is essential for the proliferation and differentiation of early hematopoietic progenitors. Flt3L is especially important for generating both conventional and plasmacytoid dendritic cells, thereby influencing antigen presentation and the initiation of adaptive immune responses.

Structurally, Flt3L exists as a type I transmembrane protein that can be cleaved to generate a soluble form, both of which are biologically active. It functions as a homodimer and binds specifically to its receptor, Flt3 (CD135), a class III receptor tyrosine kinase expressed on hematopoietic stem cells and progenitor cells. Upon ligand binding, Flt3 undergoes dimerization and autophosphorylation, activating downstream signaling pathways such as PI3K/AKT, MAPK, and STAT5, which promote cell survival and proliferation. Flt3L does not have multiple ligands but is the primary activator of the Flt3 receptor.

In disease, dysregulation of the Flt3/Flt3L axis is closely associated with hematologic malignancies, particularly acute myeloid leukemia (AML), where activating mutations in Flt3 drive uncontrolled cell growth. Therapeutically, Flt3L has been explored to enhance immune reconstitution following chemotherapy or bone marrow transplantation and to expand dendritic cells in cancer immunotherapy. Additionally, targeting mutant Flt3 with tyrosine kinase inhibitors has become an important strategy in treating AML.

Data Sheets

Recombinant Human Flt3 Ligand Protein TDS

Frequently Asked Questions

What are InnoCyto's Cytokines and Growth Factors?
These are recombinantly expressed signaling proteins, including interleukins, interferons, chemokines, and growth factors, that mediate cell communication, proliferation, differentiation, and immune activation. They are produced for use as research reagents to stimulate, modulate, or study cellular responses in vitro and in vivo.
What expression systems are these proteins produced in, and does it matter for my application?
Cytokines and growth factors may be produced in mammalian, insect, or E. coli expression systems depending on the protein, and the system affects properties like glycosylation, folding, and bioactivity. Non-glycosylated proteins from E. coli are often suitable for cell culture stimulation assays, while glycosylation-dependent proteins may require mammalian- or insect-expressed versions for full biological activity — check the product page for the expression system and recommended applications.
How is bioactivity measured and confirmed for each lot?
Bioactivity is typically confirmed using a functional cell-based assay (e.g., proliferation, differentiation, or reporter assay) relevant to the specific cytokine or growth factor, and is reported as an EC50/ED50 value or specific activity (units/mg) on the Certificate of Analysis. Always reference the lot-specific CoA rather than assuming activity is identical across lots or products.
Are these proteins carrier-free, and does that matter for my experiment?
Carrier-free formulations (without BSA or other stabilizing proteins) are available for applications where carrier protein could interfere with downstream analysis, such as certain in vivo studies or proteomic work, while carrier-containing formulations offer improved stability for routine cell culture use. Choose the format that matches your assay's sensitivity to background protein content.
Can these cytokines and growth factors be used for in vivo studies?
Mouse proteins may be used in vivo, but suitability depends on your endotoxin requirements for in vivo use. Endotoxin minimum threshholds vary by product and are indicated on the individual product page. Always confirm the endotoxin specification and formulation on the Certificate of Analysis before using a product in an in vivo setting.

Have a product or application question? Consult our FAQs or contact us.