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Recombinant human IGF1 Protein

Cat # Size Price Quantity
631201100 µg$80
6312021 mg$250

  Bulk/Custom

Product Details

ApplicationBioassay
FormatLyophilized from sterile 30% Acetonitrile, 0.1% TFA
Expression HostE.coli
Target NameIGF1, Insulin-like growth factor I, somatomedin C, Nonsuppressibleinsulin-like activity I (NSILA-I), Insulin-Like Growth Factor IB, IBP1
SpeciesHuman
accession numberP05019-1
SourcesA DNA sequence encoding the human IGF1 (P05019-1) (Gly49-Ala118) was expressed.
Molecular WeightThe recombinant human IGF1 consists of 71 amino acids and has a calculated molecular mass of 7.8 KDa.
Affinity TagNone
Purity≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Regulatory StatusRUO
Endotoxin level<0.01EU per μg protein
Protein ConcentrationLyophilized
Storage and HandlingProteins are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Research AreasCell Growth, Metabolism

Background Information

Human insulin-like growth factor 1 (IGF-1) is a peptide hormone that plays a central role in growth, development, and tissue repair. It is primarily produced by the liver in response to growth hormone stimulation, but it is also synthesized locally in many tissues where it acts in an autocrine and paracrine manner. IGF-1 is essential for postnatal growth, skeletal muscle development, and the regulation of metabolism, and it promotes cell proliferation while inhibiting apoptosis in a wide range of cell types.

Structurally, IGF-1 is a single-chain polypeptide of 70 amino acids with a three-dimensional fold similar to proinsulin, reflecting their evolutionary relationship. It contains disulfide bonds that stabilize its compact structure and allow high-affinity receptor binding. IGF-1 primarily signals through the insulin-like growth factor 1 receptor (IGF1R), a receptor tyrosine kinase that is closely related to the insulin receptor. Upon ligand binding, IGF1R activates downstream signaling pathways including PI3K/AKT and MAPK, which regulate growth, survival, and differentiation. IGF-1 also binds with high affinity to IGF-binding proteins (IGFBPs), which modulate its bioavailability and extend its half-life in circulation.

Dysregulation of IGF-1 signaling is implicated in multiple diseases. Excessive IGF-1 activity is associated with cancer progression due to enhanced cell survival and proliferation, while low levels contribute to growth failure and metabolic disorders. IGF-1 has therapeutic applications in treating growth hormone insensitivity and certain growth deficiencies. Conversely, IGF1R inhibitors and pathway modulators are being investigated as anticancer strategies to limit tumor growth driven by IGF-1 signaling.

Data Sheets

Recombinant human IGF1 Protein TDS

Frequently Asked Questions

What are InnoCyto's Cytokines and Growth Factors?
These are recombinantly expressed signaling proteins, including interleukins, interferons, chemokines, and growth factors, that mediate cell communication, proliferation, differentiation, and immune activation. They are produced for use as research reagents to stimulate, modulate, or study cellular responses in vitro and in vivo.
What expression systems are these proteins produced in, and does it matter for my application?
Cytokines and growth factors may be produced in mammalian, insect, or E. coli expression systems depending on the protein, and the system affects properties like glycosylation, folding, and bioactivity. Non-glycosylated proteins from E. coli are often suitable for cell culture stimulation assays, while glycosylation-dependent proteins may require mammalian- or insect-expressed versions for full biological activity — check the product page for the expression system and recommended applications.
How is bioactivity measured and confirmed for each lot?
Bioactivity is typically confirmed using a functional cell-based assay (e.g., proliferation, differentiation, or reporter assay) relevant to the specific cytokine or growth factor, and is reported as an EC50/ED50 value or specific activity (units/mg) on the Certificate of Analysis. Always reference the lot-specific CoA rather than assuming activity is identical across lots or products.
Are these proteins carrier-free, and does that matter for my experiment?
Carrier-free formulations (without BSA or other stabilizing proteins) are available for applications where carrier protein could interfere with downstream analysis, such as certain in vivo studies or proteomic work, while carrier-containing formulations offer improved stability for routine cell culture use. Choose the format that matches your assay's sensitivity to background protein content.
Can these cytokines and growth factors be used for in vivo studies?
Mouse proteins may be used in vivo, but suitability depends on your endotoxin requirements for in vivo use. Endotoxin minimum threshholds vary by product and are indicated on the individual product page. Always confirm the endotoxin specification and formulation on the Certificate of Analysis before using a product in an in vivo setting.

Have a product or application question? Consult our FAQs or contact us.