| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 631601 | 20 μg | $200 | ||
| 631602 | 100 μg | $610 |
| Application | Bioassay |
|---|---|
| Format | Lyophilized from sterile PBS, pH 7.4. |
| Expression Host | E.coli |
| Research Areas | Cytokines, Allergy, B cell Activation, Antibody Class Switching, Th2 Polarization, Th2 cells, Infectious Diseases |
| Target Name | IL-4,interleukin-4, B cell growth factor 1 (BCGF-1), B-cell stimulatory factor 1 (BSF-1), lymphocyte stimulatory factor 1, |
| Species | Human |
| accession number | P05112-1 |
| Sources | A DNA sequence encoding the mature form of human IL4 isoform 1 (P05112-1) (His 25-Ser 153) was expressed and purified with an initial Met. |
| Molecular Weight | The recombinant human IL4 consisting of 130 amino acids and migrates with an apparent molecular mass of 15.1 kDa as predicted. |
| Affinity Tag | None |
| Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
| Regulatory Status | RUO |
| Endotoxin level | <0.01EU per μg protein |
| Protein Concentration | Lyophilized |
| Storage and Handling | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Human interleukin-4 (IL-4) is a pleiotropic cytokine primarily produced by activated CD4⁺ T helper 2 (Th2) cells, basophils, and mast cells. It plays a central role in orchestrating humoral immunity by promoting B-cell proliferation, differentiation, and class switching to IgE and IgG4. IL-4 also suppresses Th1-mediated inflammatory responses, thereby skewing immune polarization toward a Th2 phenotype. Through these functions, IL-4 is critical in host defense against parasitic infections but also contributes to allergic inflammation.
Structurally, IL-4 is a small, secreted glycoprotein of approximately 15 kDa composed of a four α-helix bundle, a common motif among cytokines in the hematopoietin family. IL-4 signals through two receptor complexes: the type I receptor (IL-4Rα paired with the common γ chain) and the type II receptor (IL-4Rα with IL-13Rα1). Binding of IL-4 to these receptors activates the JAK-STAT pathway, particularly STAT6, leading to transcriptional regulation of target genes involved in immune modulation. IL-4 shares functional overlap with IL-13, which can bind the type II receptor complex.
Dysregulation of IL-4 is strongly associated with allergic diseases such as asthma, atopic dermatitis, and allergic rhinitis, where excessive IL-4 drives IgE production and eosinophilic inflammation. It is also implicated in tumor immune evasion and fibrosis in certain contexts. Therapeutically, targeting the IL-4/IL-4 receptor axis has proven effective; for example, monoclonal antibodies against IL-4Rα (blocking both IL-4 and IL-13 signaling) are widely used to treat moderate-to-severe atopic diseases. Conversely, recombinant IL-4 has been explored experimentally to enhance immune responses in cancer and vaccine settings, although its clinical use remains limited.
Recombinant Human IL-4 Protein TDS
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