| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 631401 | 100 µg | $550 | ||
| 631402 | 1 mg | $3150 |
| Application | Bioassay |
|---|---|
| Format | Lyophilized from sterile PBS, pH 7.4. |
| Expression Host | HEK293 |
| Target Name | MCSF, CSF1, CSF-1 |
| Species | Human |
| accession number | P09603-1 |
| Sources | A DNA sequence encoding the N-terminal fragment (Met 1-Asn 190) of human CSF1 (P09603-1) was fused with a polyhistidine tag at the C-terminus. |
| Molecular Weight | The recombinant human CSF1 consists of 169 amino acids and predictes a molecular mass of 19.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCSF1 is approximately 27-32 kDa band due to glycosylation. |
| Affinity Tag | C-His |
| Purity | > 95 % as determined by SDS-PAGE. |
| Regulatory Status | RUO |
| Endotoxin level | < 1.0 EU per μg protein |
| Protein Concentration | Lyophilized |
| Storage and Handling | Proteins are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Human macrophage colony-stimulating factor (M-CSF), also known as CSF-1, is a key cytokine that regulates the survival, proliferation, and differentiation of monocytes, macrophages, and osteoclasts. It is produced by a variety of cell types, including fibroblasts, endothelial cells, and stromal cells, and plays a central role in innate immunity, tissue homeostasis, and bone remodeling. M-CSF is essential for the development of the mononuclear phagocyte system and influences macrophage polarization and function in response to environmental cues.
Structurally, M-CSF exists as a homodimeric glycoprotein and can be found in secreted, membrane-bound, and proteoglycan-associated forms generated through alternative splicing. Its primary receptor is CSF-1R (CD115), a class III receptor tyrosine kinase expressed on monocytes and macrophages. Binding of M-CSF induces receptor dimerization and autophosphorylation, activating downstream signaling pathways such as PI3K/AKT, MAPK, and ERK, which promote cell survival and differentiation. M-CSF shares its receptor with another ligand, IL-34, which can also activate CSF-1R and modulate similar biological processes.
Dysregulation of M-CSF signaling is implicated in various diseases, including cancer, where it promotes tumor-associated macrophage development and immune suppression, as well as in inflammatory conditions and bone disorders such as osteoporosis. Therapeutically, targeting the M-CSF/CSF-1R axis with inhibitors or antibodies is being explored to modulate macrophage activity in cancer and chronic inflammation. Conversely, recombinant M-CSF has potential applications in enhancing immune recovery and tissue repair.
Recombinant Human M-CSF Protein (C-His) TDS
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