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Recombinant Human Thrombopoietin (TPO) Protein (C-His)

Cat # Size Price Quantity
63080120 μg$250
630802100 µg$580

  Bulk/Custom

Product Details

ApplicationBioassay
FormatLyophilized from sterile PBS, pH 7.4.
Expression HostCHO
Target NameTPO, THPO, Megakaryocyte Colony Stimulating Factor (MKCSF), Thrombopoietin Protein
SpeciesHuman
accession numberNP_000451.1
SourcesA DNA sequence encoding the human THPO (NP_000451.1) precursor (Met 1-Gly 353) was fused with a polyhistidine tag at the C-terminus.
Molecular WeightThe recombinant human THPO consists of 343 amino acids with the predicted molecular mass of 36.9 kDa. As a result of glycosylation, the apparent molecular mass of rhTHPO is approximately 60-80 kDa in SDS-PAGE under reducing conditions.
Affinity TagC-His
Purity> 85 % as determined by SDS-PAGE.
Regulatory StatusRUO
Endotoxin level< 1.0 EU per μg protein
Protein ConcentrationLyophilized
Storage and HandlingProteins are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Research AreasCytokines, Thrombopoiesis, Hematopoietic Stem cells, Megakaryocytes, Platelets

Background Information

Human Human Thrombopoietin (THPO) is the primary regulator of platelet production and an important factor in hematopoiesis. It is produced mainly by the liver, with additional contributions from the kidney and bone marrow stromal cells. THPO stimulates the proliferation and differentiation of megakaryocyte progenitors and promotes the maturation of megakaryocytes, ultimately leading to platelet formation.

Structurally, THPO is a glycoprotein composed of an N-terminal domain homologous to erythropoietin, responsible for receptor binding and biological activity, and a C-terminal domain that contributes to protein stability and secretion. THPO functions as a soluble ligand and exerts its effects by binding to its receptor, MPL (also known as CD110), which is expressed on hematopoietic stem cells and megakaryocyte lineage cells.

Upon binding to MPL, THPO activates intracellular signaling pathways such as JAK-STAT, MAPK, and PI3K-AKT, leading to cell survival, proliferation, and differentiation. This signaling axis is essential not only for platelet production but also for maintaining hematopoietic stem cell quiescence and self-renewal.

In disease, dysregulation of THPO or MPL signaling is associated with hematologic disorders. Reduced THPO activity can contribute to thrombocytopenia, while excessive or constitutive signaling is linked to myeloproliferative neoplasms. Mutations in MPL or downstream signaling components can disrupt normal platelet homeostasis and hematopoiesis.

Therapeutically, THPO signaling is targeted using thrombopoietin receptor agonists such as agonists such as romiplostim and eltrombopag, which are used to treat conditions like immune thrombocytopenia. These agents stimulate platelet production and represent effective treatments for patients with low platelet counts.

Data Sheets

Recombinant Human Thrombopoietin (TPO) Protein (C-His) TDS

Frequently Asked Questions

What are InnoCyto's Cytokines and Growth Factors?
These are recombinantly expressed signaling proteins, including interleukins, interferons, chemokines, and growth factors, that mediate cell communication, proliferation, differentiation, and immune activation. They are produced for use as research reagents to stimulate, modulate, or study cellular responses in vitro and in vivo.
What expression systems are these proteins produced in, and does it matter for my application?
Cytokines and growth factors may be produced in mammalian, insect, or E. coli expression systems depending on the protein, and the system affects properties like glycosylation, folding, and bioactivity. Non-glycosylated proteins from E. coli are often suitable for cell culture stimulation assays, while glycosylation-dependent proteins may require mammalian- or insect-expressed versions for full biological activity — check the product page for the expression system and recommended applications.
How is bioactivity measured and confirmed for each lot?
Bioactivity is typically confirmed using a functional cell-based assay (e.g., proliferation, differentiation, or reporter assay) relevant to the specific cytokine or growth factor, and is reported as an EC50/ED50 value or specific activity (units/mg) on the Certificate of Analysis. Always reference the lot-specific CoA rather than assuming activity is identical across lots or products.
Are these proteins carrier-free, and does that matter for my experiment?
Carrier-free formulations (without BSA or other stabilizing proteins) are available for applications where carrier protein could interfere with downstream analysis, such as certain in vivo studies or proteomic work, while carrier-containing formulations offer improved stability for routine cell culture use. Choose the format that matches your assay's sensitivity to background protein content.
Can these cytokines and growth factors be used for in vivo studies?
Mouse proteins may be used in vivo, but suitability depends on your endotoxin requirements for in vivo use. Endotoxin minimum threshholds vary by product and are indicated on the individual product page. Always confirm the endotoxin specification and formulation on the Certificate of Analysis before using a product in an in vivo setting.

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