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Recombinant Mouse GM-CSF Protein

Cat # Size Price Quantity
67010120 µg$220
670102100 μg$625

  Bulk/Custom

Product Details

ApplicationBioassay
FormatLyophilized from sterile PBS, pH 7.4.
Expression HostHEK293
Target NameGM-CSF, GMCSF, CSF Protein,
SpeciesMouse
accession numberP01587
SourcesA DNA sequence encoding the mouse CSF2 (P01587) (Ala 18-Lys141) was expressed.
Molecular WeightThe recombinant mouse CSF2 consists of 124 amino acids and predicts a molecular mass of 14.1 KDa. It migrates as an approximately 20.7 KDa band in SDS-PAGE under reducing conditions.
Affinity TagNone
Purity> 95 % as determined by SDS-PAGE
Regulatory StatusRUO
Endotoxin level< 1.0 EU per μg protein
Protein ConcentrationLyophilized
Storage and HandlingProteins are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Research AreasCytokines, Monocytes, Macrophages, Neutrophils, Eosinophils, Dendritic cells, Endothelial cells, Inflammation

Background Information

Mouse granulocyte-macrophage colony-stimulating factor (GM-CSF, encoded by Csf2) is a pleiotropic cytokine that regulates the differentiation, survival, and activation of myeloid lineage cells, particularly granulocytes, monocytes, macrophages, and dendritic cells. It is produced by activated T cells, macrophages, endothelial cells, and fibroblasts during inflammatory responses, and functions as a key mediator linking innate and adaptive immunity.


Structurally, mouse GM-CSF is a 127-amino-acid glycoprotein that adopts a four-helix bundle architecture stabilized by disulfide bonds and glycosylation, which are important for receptor binding and biological activity.


Its primary receptor is the heterodimeric GM-CSF receptor composed of a ligand-specific alpha chain (CSF2RA) and a shared beta common signaling chain (CSF2RB), which is also used by IL-3 and IL-5.


GM-CSF plays a dual role in physiology and disease: it supports host defense by enhancing macrophage and neutrophil function, but excessive signaling contributes to inflammatory and autoimmune disorders such as rheumatoid arthritis, multiple sclerosis, and pulmonary alveolar proteinosis.


Therapeutically, GM-CSF or its pathway is targeted in both directions: recombinant GM-CSF can be used to enhance myeloid recovery after chemotherapy, while monoclonal antibodies and receptor inhibitors are being developed to suppress pathological inflammation in cancer and autoimmune disease.


Downstream signaling of GM-CSF receptor activation includes JAK2/STAT5, PI3K/AKT, and MAPK pathways, which collectively regulate cell proliferation, survival, and inflammatory gene expression, making the axis a key modulatory node in immune homeostasis.

Data Sheets

Recombinant Mouse GM-CSF Protein TDS

Frequently Asked Questions

What are InnoCyto's Cytokines and Growth Factors?
These are recombinantly expressed signaling proteins, including interleukins, interferons, chemokines, and growth factors, that mediate cell communication, proliferation, differentiation, and immune activation. They are produced for use as research reagents to stimulate, modulate, or study cellular responses in vitro and in vivo.
What expression systems are these proteins produced in, and does it matter for my application?
Cytokines and growth factors may be produced in mammalian, insect, or E. coli expression systems depending on the protein, and the system affects properties like glycosylation, folding, and bioactivity. Non-glycosylated proteins from E. coli are often suitable for cell culture stimulation assays, while glycosylation-dependent proteins may require mammalian- or insect-expressed versions for full biological activity — check the product page for the expression system and recommended applications.
How is bioactivity measured and confirmed for each lot?
Bioactivity is typically confirmed using a functional cell-based assay (e.g., proliferation, differentiation, or reporter assay) relevant to the specific cytokine or growth factor, and is reported as an EC50/ED50 value or specific activity (units/mg) on the Certificate of Analysis. Always reference the lot-specific CoA rather than assuming activity is identical across lots or products.
Are these proteins carrier-free, and does that matter for my experiment?
Carrier-free formulations (without BSA or other stabilizing proteins) are available for applications where carrier protein could interfere with downstream analysis, such as certain in vivo studies or proteomic work, while carrier-containing formulations offer improved stability for routine cell culture use. Choose the format that matches your assay's sensitivity to background protein content.
Can these cytokines and growth factors be used for in vivo studies?
Mouse proteins may be used in vivo, but suitability depends on your endotoxin requirements for in vivo use. Endotoxin minimum threshholds vary by product and are indicated on the individual product page. Always confirm the endotoxin specification and formulation on the Certificate of Analysis before using a product in an in vivo setting.

Have a product or application question? Consult our FAQs or contact us.