| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 670201 | 20 μg | $140 | ||
| 670202 | 100 μg | $550 |
| Application | Bioassay |
|---|---|
| Format | Lyophilized from sterile PBS, pH 7.4. |
| Expression Host | E.coli |
| Target Name | IL-18, IL18, IL1F4, Interferon-gamma-inducing factor, IGIF, IL-1g |
| Species | Mouse |
| accession number | P70380-1 |
| Sources | A DNA sequence encoding the mouse IL18 (P70380-1) (Asn36-Ser192) was expressed and purified. |
| Molecular Weight | The recombinant mouse IL18 consists of 158 amino acids and predicts a molecular mass of 18.2 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions. |
| Affinity Tag | None |
| Purity | > 90 % as determined by SDS-PAGE |
| Regulatory Status | RUO |
| Endotoxin level | < 1.0 EU per μg protein |
| Protein Concentration | Lyophilized |
| Storage and Handling | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Research Areas | Cytokines, Inflammation, Th1 cells, NK cells, NKT cells, Wound Healing, Autoimmunity |
Mouse IL-18 is a pro-inflammatory cytokine belonging to the interleukin-1 (IL-1) family, primarily produced by macrophages, dendritic cells, and epithelial cells. It plays a central role in innate and adaptive immunity by promoting interferon-γ (IFN-γ) production, particularly in natural killer (NK) cells and T lymphocytes. IL-18 is synthesized as an inactive precursor (pro–IL-18) that requires cleavage by caspase-1 within the inflammasome complex to generate the biologically active mature cytokine.
Structurally, mature IL-18 adopts a β-trefoil fold characteristic of IL-1 family cytokines. It exerts its function by binding to a heterodimeric receptor complex composed of IL-18Rα (IL-1R5) and IL-18Rβ (IL-1R7). Upon ligand binding, this receptor complex recruits the adaptor protein MyD88, initiating downstream signaling cascades that activate NF-κB and MAPK pathways, leading to the expression of inflammatory cytokines and immune mediators. IL-18 activity is tightly regulated by IL-18 binding protein (IL-18BP), a soluble decoy that neutralizes excess cytokine.
In disease, dysregulated IL-18 production is associated with inflammatory and autoimmune conditions, including sepsis, inflammatory bowel disease, and metabolic disorders. Elevated IL-18 levels can drive excessive inflammation and tissue damage, while insufficient activity may impair host defense against infections. In cancer, IL-18 has complex roles, contributing to both anti-tumor immunity and, in some contexts, tumor progression.
Therapeutically, IL-18 is being explored as an immunomodulatory target. Recombinant IL-18 or engineered IL-18 variants are investigated to enhance anti-tumor immunity, whereas IL-18 neutralization strategies, such as IL-18BP or antibodies, are being developed to treat inflammatory diseases.
Recombinant Mouse IL-18 Protein TDS
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